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Sorbitol Metabolism in Spermatozoa - NASA/ADS

Sorbitol was identified as a chemical constituent of the seminal plasma in the ram, rabbit, bull, boar, stallion, cock and man. Its concentration was determined by an enzymic-spectrophotometric method based on the use of purified sorbitol dehydrogenase from liver. Ram spermatozoa oxidize sorbitol to fructose, and they are also capable of reducing fructose, and to a smaller …


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Spermatogenesis - an overview | ScienceDirect Topics

spermatogenesis is the process of spermatozoa (sperm) formation.12 spermatogenesis starts at puberty, when the leydig cells in the testes start to produce androgens under the influence of the follicle-stimulating hormone (fsh) and the luteinizing hormone (lh), which are in turn controlled by the gonadotrophin-releasing hormone (gnrh) produced by …


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Sorbitol Metabolism in Spermatozoa - NASA/ADS. 01-12-1959 Sorbitol dehydrogenase, the enzyme responsible for the reversible oxidoreduction of sorbitol to fructose in ram spermatozoa, was prepared in a soluble and highly active form, by grinding sperm with lavigated aluminium oxide and extraction with phosphate buffer.


The use of phosphate buffered saline for the recovery of ...

There are several methods used to recover spermatozoa and cells from the swabs before visualisation on a microscope slide and most of these methods use water. Phosphate buffered saline (PBS) is a non-toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis.


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Spermatozoon - Wikipedia

A spermatozoon (pronounced / ˌ s p ɜːr m æ t ə ˈ z oʊ ə n /, alternate spelling spermatozoön; plural spermatozoa; from Ancient Greek: σπέρμα ("seed") and Ancient Greek: ζῷον ("living being")) is a motile sperm cell, or moving form of the haploid cell that is the male gamete.A spermatozoon joins an ovum to form a zygote. (A zygote is a single cell, with a complete set …


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The Antigenic Relationship of some Mammalian Spermatozoa

Fragments of ground spermatozoa The procedure for grinding spermatozoa was repeated. 1-5 ml. of 0-85 % NaC1 was added to the sand and fragments and stirred for 10 min. to extract readily soluble material. The mixture was then centrifuged at approximately 2000 r.p.m. for 30 min., or until on microscopical examination the supernatant fluid was free


Spermatozoa Development - Embryology

This page introduces spermatogenesis the development of spermatozoa, the male haploid gamete cell. In humans at puberty, spermatozoa are produced by spermatogonia meiosis in the seminiferous tubules of the testis (male gonad). A second process of spermiogenesis leads to change in cellular organisation and shape before release into the central lumen of the …


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